FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8941138
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTT40150.018.1415161
TCAACGG27850.017.6934727
CCCGGAA20150.017.6371571
GAGACCC23900.017.6320151
CCCGAAG20900.017.5833381
GCATGAC28150.017.5830557
AACGGCC27850.017.4565059
CGGTGTG16300.017.2752674
CCAGCGC14200.017.2737456
AAGGCGA11850.017.2645327
GCCGTGA12800.017.1917921
ACGGCCT28350.017.1874310
CGGGAAG14400.017.1868883
TAACGAC21200.017.1788181
CGCTGAG17450.017.1455213
GCGCATG20750.017.1219965
CGTGAGC22000.017.0992014
CGCATGG28950.017.0647091
GGCTAGT19300.017.0406554
CGATGCG18250.017.0015051
Produced by FastQC (version 0.11.5)