FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8941138
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN188590.210923933843768No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99220.11097021430605367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT561200.073.820531
AGCAGTG635300.065.185622
CAGTGGT821650.050.3066184
GCAGTGG848000.049.017343
AGTGGTA857600.048.1804165
TGGTATC893150.046.1326037
GTGGTAT897300.045.9513556
GGTATCA915050.045.183278
GTATCAA928400.044.460829
CAACGCA915700.026.09026712-13
ATCAACG915650.026.03637110-11
ACGCAGA958450.024.97536814-15
AGAGTAC945950.024.84919518-19
GCAGAGT961350.024.73889516-17
TACATGG936100.024.57728822-23
AGTACAT950750.024.43383620-21
TCAACGC909500.022.82721912-13
CATGGGG376300.022.82536124-25
CATGGGA380350.022.63814424-25
CGCAGAG934000.022.34322416-17
Produced by FastQC (version 0.11.5)