FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8811249
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN208190.2362775129836871No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111010.1259866790735343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT547300.073.701231
AGCAGTG620050.065.055982
CAGTGGT797950.050.4325264
GCAGTGG826750.048.980193
AGTGGTA837350.048.065695
TGGTATC870600.046.093667
GTGGTAT874250.045.918576
GGTATCA892400.045.0914848
GTATCAA905200.044.3602189
ACGACGC4050.029.3798375
CAACGCA893200.026.15140312-13
ATCAACG894100.026.08899110-11
ACGCAGA937150.025.02093514-15
AGAGTAC927750.024.88319618-19
GCAGAGT939800.024.78615216-17
TACATGG917050.024.69249722-23
AGTACAT926550.024.60842520-21
CATGGGG368400.022.94179324-25
TCAACGC887450.022.76047912-13
CATGGGA375050.022.71619824-25
Produced by FastQC (version 0.11.5)