FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10206046
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN240770.23590918559449955No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113630.11133596693567714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT640100.073.713451
AGCAGTG716900.065.617352
CAGTGGT927150.050.6262634
GCAGTGG966700.048.729033
AGTGGTA971650.048.3562475
GTGGTAT1013200.046.2657626
TGGTATC1014400.046.2426347
GGTATCA1036750.045.3573238
GTATCAA1053050.044.6796849
CAACGCA1038300.026.24329212-13
ATCAACG1039500.026.16126310-11
ACGCAGA1086900.025.09085314-15
AGAGTAC1072350.025.00857218-19
GCAGAGT1090350.024.86494816-17
TACATGG1059450.024.82973722-23
AGTACAT1074000.024.700920-21
ACGACGC3650.023.2863585
CATGGGA426100.023.19583324-25
CATGGGG432450.023.14006224-25
TCAACGC1030150.022.94007712-13
Produced by FastQC (version 0.11.5)