FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10261806
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN155520.15155227062370893No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102800.10017729822606274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT646450.074.151751
AGCAGTG729000.065.639022
CAGTGGT941600.050.677224
GCAGTGG972750.049.2502673
AGTGGTA990450.048.167495
GTGGTAT1031350.046.1916166
TGGTATC1031500.046.1652037
GGTATCA1053500.045.3349388
GTATCAA1069150.044.622989
CAACGCA1055650.026.17693112-13
ATCAACG1056850.026.0993410-11
ACGCAGA1100550.025.1303614-15
AGAGTAC1088400.024.94244618-19
GCAGAGT1104250.024.86820416-17
TACATGG1072200.024.83025722-23
AGTACAT1089250.024.7080220-21
CATGGGA440750.023.11027124-25
TCAACGC1048650.022.89282412-13
CATGGGG424650.022.72112324-25
CGCAGAG1070100.022.51315516-17
Produced by FastQC (version 0.11.5)