FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9434536
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN200050.21204010456900052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT582900.073.675921
AGCAGTG656350.065.333452
CAGTGGT859150.049.779594
GCAGTGG892000.048.1260453
AGTGGTA896950.047.7126965
TGGTATC939350.045.3948557
GTGGTAT941650.045.268276
GGTATCA961750.044.519418
GTATCAA975400.043.8792049
CAACGCA960250.025.78110312-13
ATCAACG962350.025.69848810-11
ACGCAGA1004200.024.70810514-15
AGAGTAC993200.024.58284218-19
GCAGAGT1006150.024.51965516-17
TACATGG982500.024.38029122-23
AGTACAT995200.024.31000720-21
ACGACGC3900.023.9663245
CATGGGG389750.022.80003524-25
CATGGGA401850.022.58906724-25
TCAACGC953850.022.51076312-13
Produced by FastQC (version 0.11.5)