FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9351445
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAG13700.017.9848423
AAGGCGA10900.017.9621687
GAGACCC26100.017.9598981
GAGACTT41050.017.7451481
CCCGGAA19300.017.731191
ACGGAAG18100.017.6908721
ACGGCCT29400.017.5465110
AACGATG22350.017.5216452
CGGTGTG18150.017.392844
CGCATGG28150.017.3555891
TACCGGG20050.017.3423331
CGTAAGT30150.017.3356881
GTGGTAG16950.017.3263385
AACGGCC29800.017.2740759
AACGACG11850.017.266212
TCAACGG29600.017.2421537
GGGGTGA11550.017.1417735
TTGGGCG13250.017.1074831
GCACGTG22000.017.055581
GCCTACG22900.017.05132310
Produced by FastQC (version 0.11.5)