FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9317942
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN185640.19922854209652735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT574750.073.7206651
AGCAGTG645550.065.5704352
CAGTGGT842600.050.1406944
GCAGTGG872700.048.5991553
AGTGGTA882450.047.892165
GTGGTAT921400.045.7120176
TGGTATC922050.045.6239857
GGTATCA942900.044.719048
GTATCAA957000.044.0325749
CAACGCA944650.025.86277212-13
ATCAACG944050.025.84549110-11
ACGCAGA987300.024.7932714-15
AGAGTAC974100.024.72716318-19
GCAGAGT986800.024.64058316-17
TACATGG963100.024.46531922-23
AGTACAT976800.024.42247820-21
ACGACGC4200.024.2884085
CATGGGG384750.022.86356424-25
TCAACGC936450.022.60825312-13
CATGGGA392250.022.35601624-25
Produced by FastQC (version 0.11.5)