FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9260108
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT592800.074.5583041
AGCAGTG666200.066.226462
CAGTGGT872750.050.514954
GCAGTGG895750.049.2671133
AGTGGTA914100.048.2735755
GTGGTAT956650.046.0407836
TGGTATC956950.045.947347
GGTATCA976500.045.0940138
GTATCAA992550.044.4124459
ACGACGC4300.027.6952065
CAACGCA979150.025.84915512-13
ATCAACG979750.025.81925210-11
ACGCAGA1019050.024.87265814-15
AGAGTAC1012750.024.60324918-19
GCAGAGT1027650.024.50897216-17
TACATGG1000500.024.38797422-23
AGTACAT1011700.024.36971720-21
CATGGGG391400.022.89585324-25
TCAACGC972650.022.69618612-13
CATGGGA407600.022.57894724-25
Produced by FastQC (version 0.11.5)