FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9170602
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT573850.074.6560061
AGCAGTG650900.065.655632
CAGTGGT850650.050.2051734
GCAGTGG869900.049.241713
AGTGGTA882050.048.3817335
GTGGTAT924600.046.0739176
TGGTATC925050.046.0658047
GGTATCA947850.045.0594068
GTATCAA964100.044.388179
ACGACGC4250.029.0237065
CAACGCA948600.026.09469812-13
ATCAACG949150.026.03234910-11
ACGCAGA987800.025.07006314-15
AGAGTAC981900.024.86243218-19
GCAGAGT994900.024.7239516-17
TACATGG971050.024.64098222-23
AGTACAT982750.024.5603520-21
CGACGCT5300.023.274496
CATGGGA395700.022.77766624-25
TCAACGC942750.022.7667212-13
Produced by FastQC (version 0.11.5)