FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9279724
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT595750.074.35651
AGCAGTG672500.065.6998442
CAGTGGT877100.050.3419344
GCAGTGG904600.048.9274063
AGTGGTA914850.048.2651635
TGGTATC955600.046.064117
GTGGTAT956100.046.0558176
GGTATCA972900.045.3193138
GTATCAA990650.044.524969
ACGACGC3950.030.145455
CAACGCA976250.025.97392512-13
ATCAACG977250.025.92958810-11
ACGCAGA1017800.024.94167914-15
AGAGTAC1010550.024.8154318-19
GCAGAGT1024200.024.64639916-17
AGTACAT1009550.024.59319520-21
TACATGG1001450.024.54409622-23
CATGGGG398800.022.81574824-25
TCAACGC970000.022.77634612-13
CATGGGA404450.022.76971424-25
Produced by FastQC (version 0.11.5)