FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7597756
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103090.135684799564503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT480200.074.242371
AGCAGTG543050.065.5385362
CAGTGGT704950.050.499524
GCAGTGG727350.049.080753
AGTGGTA736150.048.3688285
GTGGTAT769900.046.1613166
TGGTATC769800.046.0850377
GGTATCA785750.045.2157148
GTATCAA792200.044.7777679
ACGACGC3400.027.523165
CAACGCA781250.026.26547412-13
ATCAACG782750.026.22071810-11
ACGCAGA818400.025.12488414-15
AGAGTAC811000.024.97069518-19
GCAGAGT820800.024.91506416-17
TACATGG799150.024.81343822-23
AGTACAT810550.024.73470120-21
CATGGGG319350.023.1313124-25
CATGGGA320350.023.09219724-25
TCAACGC777300.022.86413412-13
Produced by FastQC (version 0.11.5)