FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7669355
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTG15800.017.9611854
ACGGCCT23000.017.74322510
GAGACCC21400.017.742071
GAGACTT32850.017.5547581
TAACGAC18500.017.5488721
TACCGGG14750.017.5337491
CTATGAG12850.017.4622173
CCCGGAA15250.017.3918631
TCAACGG23400.017.2985617
GGGTCTG16700.017.1997811
CCTAAGA19800.017.1748221
GCATGAC21800.017.1049277
AACGGCC24000.017.0953679
GCACGTG18350.017.0925831
CGATGCG14750.017.0860791
CCCGAAG16750.017.0827371
CGCATGG23800.017.0627231
AAGGCGA9100.017.0433797
GCGCATG16050.016.996095
CGCTGAG14550.016.9339143
Produced by FastQC (version 0.11.5)