FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8920679
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT572700.074.282171
AGCAGTG642100.066.1878052
CAGTGGT833750.050.9641044
GCAGTGG860650.049.4921573
AGTGGTA875000.048.575065
TGGTATC914750.046.3622747
GTGGTAT915750.046.3477636
GGTATCA936550.045.3381168
GTATCAA951650.044.587449
CAACGCA932350.026.09249512-13
ATCAACG935300.026.009910-11
ACGCAGA975450.024.98187314-15
AGAGTAC968050.024.80347418-19
GCAGAGT980550.024.71412716-17
TACATGG956950.024.55058922-23
AGTACAT968050.024.53017220-21
ACGACGC3350.024.1231675
TCAACGC928500.022.89095712-13
CATGGGA392650.022.68107424-25
CATGGGG386450.022.52369924-25
Produced by FastQC (version 0.11.5)