FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8846533
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105550.11931227747638538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT567400.074.169961
AGCAGTG639450.065.62562
CAGTGGT832000.050.403584
GCAGTGG858350.049.0363
AGTGGTA864050.048.505535
TGGTATC902550.046.3105057
GTGGTAT903450.046.29686
GGTATCA921700.045.40968
GTATCAA940000.044.4941379
CAACGCA924650.026.0975412-13
ATCAACG926150.026.0516410-11
ACGCAGA963750.025.0762614-15
AGAGTAC954350.024.89791918-19
GCAGAGT970350.024.74186916-17
TACATGG943000.024.67950422-23
AGTACAT956100.024.6268620-21
CATGGGA390700.022.95422624-25
TCAACGC919050.022.8107612-13
CATGGGG371250.022.79763224-25
CGCAGAG941500.022.36020516-17
Produced by FastQC (version 0.11.5)