FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8957068
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT563850.074.6795351
AGCAGTG638850.065.8642352
CAGTGGT841200.049.9096954
GCAGTGG870000.048.4177253
AGTGGTA881000.047.622795
GTGGTAT923650.045.3565036
TGGTATC922850.045.356137
GGTATCA941100.044.571728
GTATCAA953850.044.0029349
ACGACGC3400.036.2717675
CAACGCA940550.025.99795712-13
ATCAACG943600.025.88419210-11
CGACGCT4800.025.6935086
ACGCAGA981000.024.9611414-15
AGAGTAC972850.024.79823518-19
GCAGAGT983650.024.7268516-17
TACATGG964600.024.54485122-23
AGTACAT974000.024.51419620-21
CATGGGA388900.023.00423424-25
CATGGGG385350.022.92444424-25
Produced by FastQC (version 0.11.5)