FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8947564
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTT39150.018.4965211
TTGGGCG13300.018.2867531
ACGGAAG16850.018.2221761
CGGGAAG12800.017.9602073
GGTGTGC13450.017.9100535
CCAGCGC16450.017.8533786
TAACGAC22400.017.7359771
GAGACCC25450.017.7293171
CGGTGTG16600.017.692054
CGCATGG27550.017.6162281
GCGCATG19550.017.6105385
AACGGCC28800.017.607129
AAGGCGA9450.017.5760067
AGAGAGT20000.017.4891918
ACGGCCT28800.017.45444510
TCAACGG28750.017.3314697
TACCGGG19450.017.3140141
GCATGAC26350.017.2820287
CCCGGAA19050.017.2731741
GGGGTGA8650.017.1669335
Produced by FastQC (version 0.11.5)