FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8043662
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT519350.074.7309651
AGCAGTG587800.065.9780042
CAGTGGT757950.050.9924664
GCAGTGG783500.049.434473
AGTGGTA790850.048.96445
TGGTATC832900.046.3562287
GTGGTAT833950.046.3332756
GGTATCA849100.045.5683988
GTATCAA863700.044.758719
CAACGCA848650.026.29085212-13
ATCAACG849900.026.27610210-11
ACGCAGA882950.025.32259614-15
AGAGTAC876600.025.18984818-19
GCAGAGT888950.025.03099616-17
TACATGG863750.024.95978722-23
AGTACAT877100.024.88449320-21
ACGACGC3650.024.471965
CATGGGG345750.023.14216224-25
CATGGGA351500.023.13755824-25
TCAACGC843900.022.97615612-13
Produced by FastQC (version 0.11.5)