FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8141831
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT523700.073.651271
AGCAGTG587500.065.550022
CAGTGGT763600.050.323824
GCAGTGG790700.048.7349743
AGTGGTA803350.047.8567245
TGGTATC835800.045.8711977
GTGGTAT837200.045.8486756
GGTATCA854550.044.939938
GTATCAA867550.044.2665149
CAACGCA851850.026.14955912-13
ATCAACG855400.026.02772310-11
ACGCAGA892050.025.04108814-15
AGAGTAC883900.024.91077218-19
GCAGAGT897000.024.76644316-17
TACATGG876750.024.62080422-23
AGTACAT885600.024.6103420-21
CATGGGG349300.023.03516824-25
CATGGGA354250.022.98867824-25
TCAACGC847650.022.70666912-13
CGCAGAG866700.022.25599916-17
Produced by FastQC (version 0.11.5)