FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9764485
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTT42350.018.266691
GGGGTGA9600.018.109855
AACGGCC28800.017.8346399
CGCTGAG16850.017.8276443
CCCGGAA20250.017.8240641
GAGACCC26500.017.4821221
GCACGTG23200.017.4549141
TAACGAC22850.017.4333271
AGGGGTG11850.017.3640984
ACGGCCT29250.017.33464410
CGTAAGT31500.017.3272271
TACCGGG20400.017.3154091
CGGGAAG13350.017.3088913
AACGACG10700.017.2765372
ATACCCG27200.017.2334372
CCTAAGA24400.017.2278651
TCAACGG29750.017.2277167
GGCTAGT18400.017.2227974
ATGGTTG38300.017.181471
ACGGAAG20800.017.1411341