FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9764485
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT624000.073.8469241
AGCAGTG702650.065.516242
CAGTGGT914450.050.2299584
GCAGTGG940350.048.9913
AGTGGTA959300.047.9608965
TGGTATC998950.045.9274677
GTGGTAT1002300.045.809736
GGTATCA1022250.044.943848
GTATCAA1036700.044.276229
CAACGCA1018800.026.02686712-13
ATCAACG1021850.025.96484610-11
ACGACGC3800.025.6706165
ACGCAGA1068200.024.86868114-15
AGAGTAC1055800.024.76023318-19
GCAGAGT1067050.024.7484816-17
AGTACAT1055950.024.55646920-21
TACATGG1045750.024.52028722-23
CATGGGA426550.022.95009824-25
TCAACGC1013500.022.7068512-13
CATGGGG419400.022.40891824-25
Produced by FastQC (version 0.11.5)