FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9737846
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT617600.073.852251
AGCAGTG698200.065.215122
CAGTGGT908850.049.9774864
GCAGTGG934700.048.8101543
AGTGGTA949600.047.8947455
TGGTATC990200.045.7505577
GTGGTAT995650.045.573196
GGTATCA1014850.044.7476968
GTATCAA1032550.044.0426649
ACGACGC4000.027.5744365
CAACGCA1010050.026.15343512-13
ATCAACG1015050.026.01095610-11
ACGCAGA1056950.025.01863514-15
AGAGTAC1042450.024.9424118-19
GCAGAGT1056150.024.88956816-17
TACATGG1028150.024.76484522-23
AGTACAT1045800.024.63763420-21
CATGGGG412600.023.07239524-25
TCAACGC1003850.022.72559712-13
CATGGGA418800.022.6699324-25
Produced by FastQC (version 0.11.5)