FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9864478
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAA21100.018.3590071
TCAACGG31600.018.3080047
TAACGAC25350.018.1462671
AACGGCC32600.018.0166829
GAGACCC26750.017.9370771
CGGGAAG15600.017.9148083
GAGACTT45800.017.8290181
AACGATG20550.017.7225342
CCCGAAG21550.017.7203041
AAGGCGA10500.017.702717
ACGGAAG18650.017.5843521
AGAGAGT21650.017.5779958
ACGGCCT33500.017.49981910
CGCTGAG19650.017.4700893
GTGGTAG18500.017.3666655
CGATGCG18750.017.3144911
CGCATGG29700.017.2670611
TGCGGAG15750.017.255183
CGGTGTG17800.017.1842294
TACCGGG21450.017.0846441
Produced by FastQC (version 0.11.5)