FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9864478
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT631200.073.918561
AGCAGTG709750.065.573432
CAGTGGT920500.050.503754
GCAGTGG946750.049.1773643
AGTGGTA964250.048.247995
TGGTATC1010700.045.9426777
GTGGTAT1011400.045.9139146
GGTATCA1030050.045.153588
GTATCAA1048250.044.4562579
CAACGCA1027100.026.21647512-13
ATCAACG1030700.026.14751410-11
ACGCAGA1073400.025.12772614-15
AGAGTAC1061700.024.97781218-19
GCAGAGT1077450.024.88102316-17
TACATGG1047650.024.80455622-23
AGTACAT1066950.024.65802220-21
ACGACGC3800.024.5617755
CATGGGG413000.023.0338624-25
CATGGGA426900.022.86137824-25
TCAACGC1022950.022.7819712-13
Produced by FastQC (version 0.11.5)