FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8078796
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84660.10479284289391638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT510150.073.908411
AGCAGTG572750.065.608522
CAGTGGT752600.049.740744
GCAGTGG776050.048.4963653
AGTGGTA787650.047.5815735
TGGTATC824350.045.3718227
GTGGTAT826200.045.31816
GGTATCA839750.044.5511638
GTATCAA851950.043.864289
CAACGCA836650.025.91542612-13
ATCAACG839600.025.83494810-11
ACGCAGA875100.024.84936114-15
AGAGTAC868800.024.70727218-19
GCAGAGT877400.024.66181216-17
AGTACAT867800.024.4964220-21
TACATGG859700.024.4809122-23
ACGACGC3150.024.2342265
CATGGGA349500.022.84171124-25
CATGGGG342450.022.54854224-25
TCAACGC832900.022.50198612-13
Produced by FastQC (version 0.11.5)