FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8091195
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102010.12607532014739478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT521250.073.660281
AGCAGTG582300.065.775032
CAGTGGT749600.050.984894
GCAGTGG776850.049.335233
AGTGGTA784500.048.7735565
GTGGTAT823650.046.3588456
TGGTATC824100.046.2823527
GGTATCA841550.045.426668
GTATCAA854350.044.6461569
CAACGCA837700.026.14934312-13
ATCAACG840550.026.0731410-11
ACGCAGA871850.025.11033814-15
AGAGTAC867450.024.9465718-19
TACATGG856050.024.79294422-23
GCAGAGT881950.024.77812616-17
AGTACAT867550.024.70958720-21
CATGGGA348850.023.3463224-25
TCAACGC833400.022.93723112-13
CATGGGG345350.022.51209324-25
CGCAGAG852950.022.49119216-17
Produced by FastQC (version 0.11.5)