FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8134584
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102480.12598062789689063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT518700.074.235831
AGCAGTG584800.065.843412
CAGTGGT762350.050.507234
GCAGTGG783150.049.424363
AGTGGTA795600.048.4714245
TGGTATC828650.046.3960387
GTGGTAT831650.046.3105476
GGTATCA844550.045.5379838
GTATCAA856250.044.8515939
ACGACGC4000.027.5759225
CAACGCA838250.026.31819512-13
ATCAACG843650.026.16278510-11
ACGCAGA876500.025.21367314-15
AGAGTAC870550.025.00670218-19
GCAGAGT883100.024.93198216-17
TACATGG860050.024.81012522-23
AGTACAT869950.024.77277820-21
TCAACGC835100.023.0186112-13
CATGGGA341400.022.96551324-25
CATGGGG351300.022.69946924-25
Produced by FastQC (version 0.11.5)