FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9424270
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACCC27800.018.0504281
CCCGGAA18850.018.0390871
CCAGCGC17300.017.8725346
TCAACGG29050.017.83217
CGGTGTG17450.017.7189034
TAACGAC23000.017.7027741
GCATGAC28750.017.673887
GCACGTG21500.017.6070821
CGATGCG18500.017.6070821
AACGGCC29750.017.5975559
ACGGCCT29650.017.58271810
CGCTGAG17400.017.58023
GAGACTT42400.017.5447941
CGGGAAG13800.017.5417063
ACGGAAG17250.017.4157031
CGCATGG29600.017.3245371
ACTAGCG11750.017.3244635
AGCGCCG7700.017.2833128
ATACCCG25650.017.2023412
TACCGGG20100.017.1909941
Produced by FastQC (version 0.11.5)