FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9424270
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT589700.074.13371
AGCAGTG667300.065.373622
CAGTGGT876500.049.600944
GCAGTGG906450.048.1516083
AGTGGTA917050.047.485785
TGGTATC956900.045.352377
GTGGTAT961450.045.2018176
GGTATCA980500.044.3183638
GTATCAA994750.043.6564679
CAACGCA976950.025.78427712-13
ATCAACG982000.025.63809610-11
ACGCAGA1020100.024.73708214-15
AGAGTAC1009100.024.65224618-19
GCAGAGT1028450.024.45098116-17
TACATGG999800.024.4090222-23
AGTACAT1014350.024.3414420-21
CATGGGA407200.022.72594324-25
TCAACGC972950.022.38598812-13
CATGGGG400250.022.35589224-25
ACGACGC4600.022.1249285
Produced by FastQC (version 0.11.5)