FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9407847
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105180.11180028756845217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT599950.073.91751
AGCAGTG667750.066.238822
CAGTGGT878300.050.3524864
GCAGTGG902450.049.123793
AGTGGTA915300.048.305185
GTGGTAT960650.045.9308136
TGGTATC959300.045.8806467
GGTATCA977200.045.067658
GTATCAA991850.044.3926439
ACGACGC3850.026.4432585
CAACGCA973400.026.03648212-13
ATCAACG977450.025.90920610-11
ACGCAGA1017450.024.94621814-15
AGAGTAC1011900.024.8102918-19
GCAGAGT1022300.024.78356616-17
TACATGG996650.024.683222-23
AGTACAT1010800.024.61770620-21
CATGGGA399850.022.92051924-25
CATGGGG404050.022.76643224-25
TCAACGC968900.022.76549712-13
Produced by FastQC (version 0.11.5)