FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9528309
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT599350.074.27031
AGCAGTG678600.065.446472
CAGTGGT886450.050.0348554
GCAGTGG913500.048.6273353
AGTGGTA934500.047.476185
TGGTATC972600.045.5454867
GTGGTAT976550.045.4058386
GGTATCA990350.044.7488568
GTATCAA1007250.043.9951639
ACGACGC3800.029.0266045
CAACGCA986750.025.9852712-13
ATCAACG991050.025.92079410-11
ACGCAGA1032300.024.88706214-15
AGAGTAC1018850.024.82882118-19
TACATGG1007850.024.6224322-23
GCAGAGT1036900.024.61813716-17
AGTACAT1022300.024.5628620-21
CATGGGA408250.023.10937924-25
TCAACGC982350.022.61567112-13
CATGGGG406700.022.3717724-25
Produced by FastQC (version 0.11.5)