FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8657152
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT549700.074.091171
AGCAGTG616100.066.004712
CAGTGGT800050.050.7562034
GCAGTGG821350.049.5512733
AGTGGTA835400.048.6594775
TGGTATC868650.046.6599857
GTGGTAT873200.046.454456
GGTATCA887900.045.6844678
GTATCAA899700.045.1156779
CAACGCA883200.026.31616412-13
ATCAACG885000.026.27987310-11
ACGACGC3850.025.3438665
ACGCAGA924950.025.20226114-15
AGAGTAC917350.025.07728818-19
TACATGG905000.024.91794822-23
GCAGAGT931000.024.89264916-17
AGTACAT921050.024.7760120-21
CATGGGA371100.023.15578524-25
TCAACGC879050.023.1220612-13
CATGGGG358500.023.0210424-25
Produced by FastQC (version 0.11.5)