FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8588773
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAA18900.018.2240641
AGGGGTG9500.017.8314974
GCACGTG20500.017.821611
GAGACTT37400.017.8010861
GCGCATG18750.017.7172555
GAGACCC24950.017.6421761
ACGGCCT26600.017.57459810
CGGGAAG10550.017.5166113
CGGTGTG16600.017.4938984
ACGGAAG17250.017.4155121
AACGATG18850.017.3901462
ATACCCG22500.017.306892
TCAACGG27200.017.26777
GTGGTAG16800.017.2201615
CGCATGG24950.017.1570151
GCATGAC25650.017.1104357
TAGCGGA10000.017.04942510
AAGGCGA10350.017.004167
CGTAAGT24550.016.988321
TAACGAC20550.016.9750141
Produced by FastQC (version 0.11.5)