FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8588773
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT547150.073.746381
AGCAGTG617150.065.2439042
CAGTGGT801150.050.1530344
GCAGTGG820200.049.1169783
AGTGGTA839250.047.9368135
GTGGTAT876400.045.864416
TGGTATC876000.045.8521737
GGTATCA891300.045.0613068
GTATCAA906700.044.3242539
CAACGCA890400.026.10642812-13
ATCAACG893000.026.03747410-11
ACGCAGA931900.025.00271814-15
AGAGTAC922850.024.87621518-19
GCAGAGT937500.024.72428516-17
TACATGG911100.024.69056522-23
AGTACAT924150.024.60212920-21
ACGACGC3650.024.4136545
CATGGGG362100.023.02014724-25
TCAACGC884800.022.73467412-13
CATGGGA369150.022.72444324-25
Produced by FastQC (version 0.11.5)