FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11490411
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG116780.10163256997508618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT719100.073.624941
AGCAGTG808700.065.3599552
CAGTGGT1050800.050.2292024
GCAGTGG1083400.048.793313
AGTGGTA1095400.048.2470175
TGGTATC1140450.046.1837437
GTGGTAT1145000.046.0837256
GGTATCA1163800.045.3572128
GTATCAA1183050.044.6294829
ACGACGC4500.026.3601635
CAACGCA1159950.026.22648412-13
ATCAACG1167150.026.09606710-11
ACGCAGA1214150.025.07708714-15
AGAGTAC1204000.024.97892818-19
GCAGAGT1220450.024.84939216-17
AGTACAT1202950.024.76843320-21
TACATGG1188150.024.7608622-23
CATGGGG472500.022.99951424-25
TCAACGC1154650.022.96651812-13
CATGGGA480450.022.84013224-25
Produced by FastQC (version 0.11.5)