FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11580898
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG119270.10298855926371168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT720300.073.3208851
AGCAGTG803100.065.597582
CAGTGGT1046550.050.132144
GCAGTGG1079850.048.7317663
AGTGGTA1092300.048.129835
TGGTATC1137350.046.0828557
GTGGTAT1142350.045.9143566
GGTATCA1165900.045.0636758
GTATCAA1184300.044.410089
CAACGCA1162100.026.09454312-13
ATCAACG1166700.026.03543510-11
ACGCAGA1216650.024.9701614-15
AGAGTAC1204850.024.88178818-19
GCAGAGT1217450.024.7704916-17
AGTACAT1204100.024.69103420-21
TACATGG1189800.024.65165122-23
CATGGGA484900.023.14526724-25
TCAACGC1154200.022.8456612-13
CATGGGG474400.022.51969124-25
CGCAGAG1180750.022.36493916-17
Produced by FastQC (version 0.11.5)