FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9541289
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG112210.11760465488468068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT596300.073.298131
AGCAGTG670400.065.109322
CAGTGGT875450.049.844744
GCAGTGG902050.048.626833
AGTGGTA911500.047.990835
TGGTATC945500.046.084627
GTGGTAT950950.045.8502586
GGTATCA966250.045.1312338
GTATCAA980000.044.3799
CAACGCA960400.026.19051212-13
ATCAACG964650.026.08064510-11
ACGCAGA1004050.025.1049414-15
AGAGTAC998100.024.91870318-19
GCAGAGT1016000.024.7747116-17
TACATGG987950.024.72018822-23
AGTACAT999450.024.67311920-21
CATGGGA399950.022.96646324-25
TCAACGC955500.022.80315812-13
CATGGGG395850.022.73187324-25
CGCAGAG981250.022.32252316-17
Produced by FastQC (version 0.11.5)