FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9626866
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113780.1181900734880905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT592650.073.933681
AGCAGTG668450.065.443762
CAGTGGT870600.050.132264
GCAGTGG896650.048.881253
AGTGGTA914700.047.7623445
TGGTATC952700.045.733947
GTGGTAT957350.045.5819446
GGTATCA972650.044.7968258
GTATCAA985150.044.129799
CAACGCA965100.025.9641712-13
ATCAACG968750.025.88268510-11
ACGCAGA1012850.024.73541614-15
AGAGTAC1004300.024.66101818-19
GCAGAGT1018200.024.6042116-17
TACATGG993450.024.53322622-23
AGTACAT1006300.024.41676120-21
TCAACGC960650.022.66657312-13
CATGGGG400400.022.61225324-25
CATGGGA405950.022.41828524-25
CGCAGAG987000.022.18529516-17
Produced by FastQC (version 0.11.5)