FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10032391
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTT42950.017.8567091
ACGGCCT30700.017.62816210
GCGCATG21700.017.592345
CCCGAAG22450.017.5468241
CGCATGG29450.017.5234411
CGTAAGT30100.017.437481
TAACGAC23100.017.4341561
AGGGGTG12150.017.385084
AGAGAGT22800.017.3679248
CCCGGAA21400.017.3279821
GAGACCC27400.017.2683051
CGGGAAG13650.017.2477663
CGGTGTG18950.017.242454
GCGGTCG9450.017.2298554
GGGGTGA10350.017.2198185
ACGGAAG21050.017.1456361
ATACCCG25900.017.1183282
AACGGCC31750.017.1144719
TCAACGG32150.017.1068237
CGCTGAG19300.017.1007693