FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10110465
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105290.10413962166923084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT621000.073.647471
AGCAGTG694450.065.7612
CAGTGGT907450.050.314134
GCAGTGG934150.048.9863173
AGTGGTA953750.047.9120835
TGGTATC991450.046.0030067
GTGGTAT994650.045.892456
GGTATCA1013850.045.037468
GTATCAA1032650.044.1657879
CAACGCA1010050.025.86446212-13
ATCAACG1017000.025.74774710-11
ACGCAGA1058300.024.75241314-15
AGAGTAC1048400.024.69470618-19
GCAGAGT1062100.024.59920916-17
AGTACAT1046050.024.48627320-21
TACATGG1038400.024.41982722-23
ACGACGC3750.023.7338565
CATGGGA421950.022.8919224-25
TCAACGC1006100.022.66281312-13
CATGGGG410350.022.42553524-25
Produced by FastQC (version 0.11.5)