FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10019768
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT616900.073.538331
AGCAGTG691050.065.508342
CAGTGGT901850.050.12454
GCAGTGG931550.048.583323
AGTGGTA939050.048.2704545
GTGGTAT983050.045.9556966
TGGTATC983400.045.885147
GGTATCA1004900.045.052638
GTATCAA1022300.044.2541929
CAACGCA1000200.026.05492812-13
ATCAACG1004050.025.98678410-11
ACGCAGA1047000.024.93324314-15
AGAGTAC1038650.024.87961218-19
GCAGAGT1052600.024.74621416-17
AGTACAT1039250.024.66014720-21
TACATGG1026900.024.6536822-23
CATGGGA417050.022.81167224-25
TCAACGC993750.022.80035812-13
ACGACGC3550.022.6837355
CATGGGG405700.022.59589824-25
Produced by FastQC (version 0.11.5)