FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10416344
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN236760.2272966407407436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT664550.074.156611
AGCAGTG746050.066.0176852
CAGTGGT968500.050.6921654
GCAGTGG992400.049.585013
AGTGGTA1008600.048.7123155
TGGTATC1048300.046.68817
GTGGTAT1050400.046.6262176
GGTATCA1074150.045.640998
GTATCAA1092850.044.8647779
ACGACGC4300.030.6270585
CAACGCA1071150.026.3218912-13
ATCAACG1073600.026.27268210-11
ACGCAGA1118450.025.23700114-15
AGAGTAC1111650.025.12671918-19
GCAGAGT1128900.024.94800416-17
TACATGG1100750.024.82197822-23
AGTACAT1117350.024.79362720-21
TCAACGC1064950.023.06574212-13
CATGGGG433500.023.05888224-25
CATGGGA445100.023.04038624-25
Produced by FastQC (version 0.11.5)