FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8641972
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN166110.19221307359014816No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99710.1153787584592961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT543900.074.189581
AGCAGTG615150.065.4681242
CAGTGGT796500.050.538334
GCAGTGG820750.049.1802863
AGTGGTA832000.048.4338465
TGGTATC866750.046.351247
GTGGTAT873100.046.090646
GGTATCA885400.045.450838
GTATCAA900500.044.567059
ACGACGC2650.027.2516125
CAACGCA882950.026.25846712-13
ATCAACG887850.026.11119810-11
ACGCAGA922850.025.16533514-15
AGAGTAC917600.024.98329418-19
GCAGAGT928200.024.95299716-17
TACATGG901650.024.93880322-23
AGTACAT917750.024.7387120-21
CATGGGA365550.023.25666224-25
TCAACGC878750.022.88319212-13
CATGGGG359550.022.64601924-25
Produced by FastQC (version 0.11.5)