FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8654135
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN117380.13563458393010971No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98850.11422285416162331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT549250.073.534261
AGCAGTG611550.065.9638062
CAGTGGT803950.050.142744
GCAGTGG826750.048.8892633
AGTGGTA840300.048.0727735
TGGTATC874350.045.9873967
GTGGTAT880000.045.773686
GGTATCA893750.045.0205738
GTATCAA905950.044.3452349
ATCAACG890950.026.02073110-11
CAACGCA891000.025.99208812-13
ACGCAGA932350.024.9173914-15
GCAGAGT939050.024.69295116-17
AGAGTAC929350.024.67932718-19
TACATGG916100.024.53340522-23
AGTACAT926950.024.49591820-21
TCAACGC885300.022.75871512-13
CATGGGA374450.022.64269624-25
CATGGGG367650.022.56442624-25
CGCAGAG910950.022.25212116-17
Produced by FastQC (version 0.11.5)