FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8653143
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAA19550.018.4062541
GAGACTT36950.017.898821
GCACGTG21400.017.8949241
ACGGCCT25700.017.76217310
GGGCTAG13150.017.732963
CGCATGG25050.017.6596761
GCATGAC28400.017.6223647
GTGGAGA14650.017.5769141
ACGGAAG16100.017.565951
CCCGAAG18400.017.523231
GGCTAGT20600.017.5136934
CGGTGTG16350.017.4914864
GCGCATG19900.017.3559235
TCAACGG26300.017.314737
AGGGCGT7250.017.2956943
TAACGAC22600.017.2855951
GAGACCC22750.017.2683661
AACGGCC26700.017.2614159
AGGGGTG11800.017.2449074
CGCATGA21700.017.2341676
Produced by FastQC (version 0.11.5)