FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8653143
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN166740.19269299028110365No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97780.11299940380044568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT541100.074.02521
AGCAGTG610550.065.439672
CAGTGGT796850.050.1737944
GCAGTGG824350.048.607393
AGTGGTA833750.047.9293985
TGGTATC864200.046.051557
GTGGTAT870350.045.8221866
GGTATCA880100.045.1959658
GTATCAA893350.044.51119
CAACGCA878450.026.17452212-13
ATCAACG882700.026.04533810-11
ACGCAGA920300.025.0867914-15
AGAGTAC916250.024.95150618-19
GCAGAGT927300.024.83011616-17
AGTACAT916750.024.6692420-21
TACATGG906150.024.6672522-23
CATGGGA374000.023.08556624-25
TCAACGC874150.022.84494212-13
CATGGGG359800.022.75047124-25
CGCAGAG898650.022.42492516-17
Produced by FastQC (version 0.11.5)