FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10008436
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGG31550.017.9883567
GAGACTT44250.017.9290081
AACGGCC31550.017.9186339
ACGGAAG17550.017.8063741
CGCATGG31950.017.736611
CGGGAAG14950.017.4418073
TAACGAC23350.017.341841
AGAGAGT21900.017.326718
GAGACCC27000.017.320461
CCCGGAA19950.017.3189281
ACGGCCT32600.017.2745410
GGTGTGC14150.017.2615935
GCACGTG24750.017.2500671
AAGGCGA12650.017.2153477
CGGTGTG17100.017.1790624
AACGATG24400.017.1359622
CGATGCG21150.017.116691
CGCTGAG19100.017.108323
CGTGAGC26150.017.0399254
AATCGTG40150.016.9918381
Produced by FastQC (version 0.11.5)