FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10024199
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN227560.22701065691134023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT634950.073.6006551
AGCAGTG707450.065.8437962
CAGTGGT914650.050.8919564
GCAGTGG942050.049.4964263
AGTGGTA954100.048.9177785
TGGTATC1000950.046.4750867
GTGGTAT1003250.046.400796
GGTATCA1022350.045.595058
GTATCAA1038700.044.8791479
ACGACGC3850.030.8943315
CAACGCA1019600.026.19182212-13
ATCAACG1021250.026.13304310-11
ACGCAGA1065200.025.0882514-15
AGAGTAC1058750.024.9609318-19
GCAGAGT1072350.024.82059916-17
TACATGG1046950.024.7640822-23
AGTACAT1061150.024.66492520-21
CATGGGA426150.023.14720724-25
TCAACGC1013250.023.04731612-13
CATGGGG422200.022.79502324-25
Produced by FastQC (version 0.11.5)