FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10023684
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAG15150.018.078393
TCAACGG28700.017.8983237
GAGACTT42500.017.8129161
ACGGCCT28250.017.79432710
CGATGCG20550.017.7235891
CGGTGTG18550.017.6703594
TTGGGCG14400.017.5752221
GAGACCC28300.017.5746821
CGCATGG31200.017.5634671
TAACGAC25500.017.5626371
AACGGCC28800.017.5308049
TAGCGGA13650.017.48682410
GCGCATG22100.017.4697975
GGGGTGA12100.017.4537545
GGCTAGT21950.017.3887334
ACGGAAG19450.017.1984581
GCCGTGA14100.017.1687641
CGTAAGT33250.017.1093851
AGGGGTG14150.017.1016964
AAGGCGA11900.017.1002357
Produced by FastQC (version 0.11.5)