FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10023684
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN191260.19080809011936128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT628950.074.251941
AGCAGTG711700.065.497522
CAGTGGT921550.050.5524
GCAGTGG955850.048.873813
AGTGGTA965150.048.3749585
TGGTATC1012000.045.931137
GTGGTAT1013650.045.923436
GGTATCA1033050.045.087058
GTATCAA1049000.044.385979
CAACGCA1029950.026.18103812-13
ATCAACG1032150.026.07106210-11
ACGCAGA1074500.025.17963614-15
AGAGTAC1072150.024.88701818-19
GCAGAGT1084450.024.849816-17
TACATGG1052200.024.78938322-23
AGTACAT1069750.024.73819420-21
CATGGGA427250.023.1068524-25
TCAACGC1022750.022.81728612-13
CATGGGG417400.022.58305524-25
CGCAGAG1050050.022.39569916-17
Produced by FastQC (version 0.11.5)